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Cell viability evaluated by MTT assay Viability of the cells was assayed using cell proliferation Kit I with the absorbance of 595 nm being detected using a Perkin-Elmer Victor plate reader

It remains unclear if this approach will generalize to all organs in the body, such as reproductive organs, and future studies should address this question

Oxidative stress is involved in the process of aging (Kregel and Zhang 2007) and various chronic diseases such as atherosclerosis (Fearon and Faux 2009), diabetes (Ceriello and Motz, 2004) and eye disease (Li et al

PMID: 17925621

doi: 10.1016/S1535-6108(03)00050-3 12 LeiGZhuangLGanB

Hypoxia stimulates low-density lipoprotein receptorrelated protein-1 expression through hypoxia-inducible factor-1 in human vascular smooth muscle cells