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hESCs line MAN13 (Ye et al, 2017) was maintained in feeder-free culture conditions in 6-well plates pre-coated with Matrigel matrix (Corning) in mTeSR1medium (Stem Cell Technologies)

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In roots of seedlings that were exposed to AITC for 72 h roGFP2 fluorescence was almost completely absent (data not shown)

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