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Maximal recombination levels are reached by 3 or 5 days of TAM injections in cerebellum or cortex, respectively To determine the in vivo efficacy of TAM injections to activate the Cre DNA recombinase, we studied the recombination of two floxed gene loci, p2ry1 (P2Y1 receptor) and gria1 (AMPA receptor subunit GluA1) in two different areas of the brain, cerebellum (cb) and cortex (ctx), using GLAST CreERT2 mice (high-affinity glutamate/aspartate transporter, GLAST, slc1a3

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All samples, blank, and/or MDA standard solutions were mixed with 400 l of TBA solution and then vortexed for 1 min

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The supplement was well-tolerated, with no significant treatment-related adverse events, including liver toxicity, compared to placebo