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To differentiate monocytes into the M1 and M2 phenotypes, RosetteSep was used by Ficoll isolationIMDM 10% FBS +50ng/ml human M-CSF (for M2 macrophages) or 50ng/ml GM-CSF (Biolegend, San Diego, Calif.) (for M1 macrophages)

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Responses of Mycobacterium tuberculosis to growth in the mouse lung

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