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The relationship between basal metabolic rate (BMR) and concentrations of plasma thyroid hormones in fasting cockerels

Coating of the target protein on magnetic beads for the aptamers selection Saturation of the beads surface was performed using 200 g of CspZ, either conjugated to His Tag or fused to GST, immobilized on each type of beads (160 L of beads solution were used for HisPur Ni-NTA Magnetic Beads, whereas 50 L were used for the Glutathione High Capacity Magnetic Agarose Beads)

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Fluorescence intensity was quantified by using the ImageJ software (NIH)