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Although we cannot rule out a contribution of glycolysis to this ATP increase and did not directly assess the activity of other complexes, we observed increased 5FU sensitivity with inhibitors of ETC complexes IIV, genetic ablation of CI and CIV, and sensitizer genes from CRISPR screening covering additional complexes (CIII and CV)

Theoretical concerns include growth factor-related effects in individuals with existing malignancies and immune modulation in vulnerable populations

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Int J Mol Sci (2019) 20(23):6003

For experiments in 384-well plates, 500 cells were seeded per well, and after 24 h, the medium was switched using a series of 4 aspirationwash steps with the desired final medium using Multidrop Combi (Thermo)