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While ROS easily damages DNA, the regulated oxidation of sulfhydryls to disulfide in protamines is required for sperm maturation in the epididymis (Figure 3) [91]

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In addition, pyruvate and malate/glutatamate, which generate Complex I substrate NADH, produce very little O 2 when added to reaction chambers (Muller et al., 2008)

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Competitive elution: Selective eluents are often used to separate substances on a group specific medium or when the binding affinity of the ligand/target protein interaction is relatively high