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After 16 h of growth, cells were harvested via centrifugation and the resulting supernatant was harvested and sterilized using 0.22 m syringe filter sterilizing units (VWR, 76479-016) to remove residual cells

However, this skin disorder is common in people with diabetes who inject insulin subcutaneously every day

Minn Med

A549 cells were incubated with pneumococci for 3 hours at 37C and 5% CO2 to allow bacterial internalization, while the other cell lines were incubated for only 1 h with bacteria

An D, Schneller JL, Frassetto A, Liang S, Zhu X, Park JS, Theisen M, Hong SJ, Zhou J, Rajendran R, et al

DNA synthesis: B12 plays a direct role in cellular replication and repair