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However, its impact was neutralised by performing trim and fill analysis

For method 3, magnetically enriched cultures at 90% parasitaemia and 0.12% Hct were incubated with the test compounds for the desired times (drug treatments, 100 nM DHA for 3 h, 300 nM OZ277 for 3 h, 300 nM OZ439 for 6 h, 1 M pyrimethamine for 5 h, and three control conditions, 1 mM DIAM for 5 min, 10 mM DTT for 5 min and DMSO for 6 h)

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Therefore, H 2 O 2 treatment of WT cells during spontaneous DE differentiation recapitulates the cell fate bias observed in GPX2-deficient PEN, suggesting that H 2 O 2 induced oxidative stress impacts the cell fate decisions regardless of the in vitro differentiation approach