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Severe complications of a caffeine overdose are rare, but can include: A healthcare provider will diagnose a caffeine overdose with a physical exam and some tests

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F 2 -isoprostanes Isoprostanes are specific prostaglandin-like molecules formed by the non-enzymatic ROS-mediated peroxidation of PUFAs, involving for example arachidonic acid (Milne et al

Phys Rev Lett 23:868-871, 1969

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However, its clinical applicability is severely constrained by poor stability and susceptibility to oxidation, limiting its translational potential