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doi: 10.1371/journal.pone.0009836 65 LeyREBckhedFTurnbaughPLozuponeCAKnightRDGordonJI

2023;120(9):e2214165120

For microscopic detection of cell death and NET formation, neutrophils (400 L, 5 10 6 /mL) in RPMI supplemented with 0.5% human serum albumin were seeded into cell-culture dishes with cover glass bottom (MoBiTec) for 30 min

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Spermine directly binds to JAK1 and inhibits its activity, alleviating autoimmune pathogenesis in SLE mouse models while reducing excessive IFN-I signaling activation in SLE patient monocytes [59]