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Briefly, total RNA from cells was isolated using a Pure link RNA Mini kit (Life Technologies, USA), according to the manufacturers protocols

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Additionally, HD has been shown to protect against Valproate-induced hepatotoxicity by its scavenging of free radicals, antioxidant property and prevent oxidative stress mediated apoptosis, as assessed by liver marker enzymes, oxidative stressors, antioxidant enzymes, mRNA and protein expression of apoptotic proteins (Nathiya et al., 2016)

Evidence also exists for c-MET interaction with the other EGFR family members ERBB2 and ERBB3 (for erythroblastic leukemia viral oncogene homologs B2 and B3), causing transactivation of both receptors [Bachleitner-Hofmann et al