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Description

doi: 10.3390/v10120712

Gustafsson B, Dorn GW

NAD(+)-boosting molecules suppress mast cell degranulation and anaphylactic responses in mice

Cells lysed in GST lysis buffer (50 mM Tris pH 8.0, 150 mM NaCl, 0.05% Triton X-100, 2 mM DTT)

Assay: GSH/GSSG in mice tissues

This heightened membrane tension activates Piezo1 and transient receptor potential (TRP) mechanosensitive ion channels, while simultaneously inactivating membrane Na+/K+-ATPases