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polynucleotide shuffling & interrupted synthesis non-stochastic polynucleotide reassembly methods of the invention Optimal plasmid binding to and uptake by the chosen antigen expressing cells (e.g., myocytes for intramuscular immunization or epithelial cells for mucosal immunization) Optimal vector binding to the target cell includes not only the concept of very avid binding and subsequent intemalization into target cells, but relative inability to bind to and enter other cells