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These physiological processes prevent oral doses from resulting in therapeutic plasma concentrations sufficient for significant antioxidant effects in tissues, and low solubility combined with rapid metabolism reduces the time in which the antioxidant remains in its active form, compromising its efficacy in chronic diseases that require sustained action [57, 58]

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This was achieved using the Matlab scripts given in the supplementary software of the Tsigkinopoulou et al protocol

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Changchien, C.Y., Y.H

The influence of fasting and physical exercise on liver function To assess liver function, it is important to determine the levels of aminotransferases such as AST and ALT, the activity of which in our studies significantly increased under the influence of 8 days of water-only fasting, without the participation of physical exercise