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To view a copy of this licence, visit About this article Cite this article Mndez-Ruette, M., Bedoya, M., Hinrichsen, B

Measurement of hemoglobin at each phlebotomy is recommended, whereas serum ferritin can be measured at every fourth phlebotomy during the early induction phase but increased to every phlebotomy when the serum ferritin reaches 200 g/L

Quantification of the sample was done using the same parameters as the GPF library except that the match between run option was enabled

An isozyme not retained on the affinity column has the highest activity toward 1,2-dichloro-4-nitrobenzene

ALT: alanine transaminase AST: aspartate transaminase DMT1: divalent metal transporter 1 RBC: red blood cell count FAC: ferric citrate FTH/L: ferritin heavy/light chain Fpn: ferroportin GSH: glutathione GSSG: glutathione, Oxydized GPX4: glutathione peroxidase 4 HGB: hemoglobin HCT: hematocrit HO-1: heme oxygenase 1 MCV: mean corpuscular volume MCH: mean corpuscular hemoglobin MCHC: mean corpuscular hemoglobin concentration MDA: malondialdehyde NOQ1: quinone oxidoreductase 1 Ptgs2: Prostaglandin G/H synthase 2 ROS: reactive oxygen species SLC3A2: solute carrier family 3 member 2 SLC7A11: solute carrier family 7 member 11 TF: transferrin saturation TIBC: total iron binding capacity TfR1: transferrin receptor 1 UIBC: unsaturated iron binding capacity The authors have declared that no competing interest exists

Add 0.2 ml of Elution Buffer and centrifuge