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(2024) using a Mini-PROTEAN Bio-Rad system

After 10 consecutive days of intraperitoneal injection, the mice in each group were weighed and then euthanized with an overdose of 3% pentobarbital sodium (180 mg/kg)

These problems are caused by various phytopathogenic organisms, such as microorganisms and insects, each with different infection, development, and survival strategies within the host (Meng et al., 2009

These results demonstrate that multi-analyte sensing can improve the early diagnostic sensitivity of kidney disease by capturing the interconnected metabolic changes in the body

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The PVDF membrane was then subjected to blocking with 5% milk and subsequently incubated with the designated primary antibodies overnight at a temperature of 4 C: -actin (Proteintech, 66009-1-lg) (1:50,000), SIRT1 (Abcam, ab189494) (1:1000), NF-B p65(CST, 8242) (1:10,000), NF-B p65 (acetyl Lys310) (Abcam, ab19870) (1:10,000), Type II Collagen Alpha 1 Chain (COL2A1) (Proteintech, 28459-1-AP) (1:1000), Ferritin heavy chain 1 (FTH1) (Abcam, ab183781) (1:1000), Glutathione peroxidase 4 (GPX4) (Abcam, ab125066) (1:1000), acyl-CoA synthetase long-chain family member 4 (ACSL4) (Proteintech, 22401-1-AP) (1:5000), P300 (Santa Cruz, sc-48343)(1:1000)