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Immediately thereafter, cardiac perfusion was performed with 20 mL of ice-cold phosphate-buffered saline (PBS) to remove residual blood

Usually such buffer should have pH of around 7.0 - 8.0 to facilitate efficient interaction between GST and its substrate glutathione, protease inhibitors (such as PMSF) to prevent protein degradation

cDNA was synthesized using Superscript III First-Strand Synthesis System (Invitrogen, Carlsbad, CA) according to manufacturers instructions using 1 g total RNA in a 20 l reaction volume

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Abstract Oxalate homeostasis is maintained through a delicate balance between endogenous sources, exogenous supply and excretion from the body

It discusses the various uses of SI seeds and oil, both traditionally and commercially