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post-treatment, halving required *n*) Accept reduced power (e.g., 0.7) with explicit disclosure Sensitivity Validation Verify calculations using the WebPower online tool: Method: Input actual sample size Determine minimum detectable fold change Confirm alignment with experimental objectives Trap 10: Isotopic Labels Impair Tyrosine Phosphorylation Detection Mechanistic Basis TMT/iTRAQ reagents target peptide N-termini but react with phosphotyrosine (pY) groups

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