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To further support this model, we monitored the precursor of miR-4488 (pre-miR-4488)

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Irisin activates SIRT1 to deacetylate HIF-1, reducing lactate metabolism and cell apoptosis To explore how irisin regulates HIF-1 through SIRT1, we used co-immunoprecipitation to isolate HIF-1 and measured its acetylation status

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