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The membranes were stained with 0.5% Ponceau S (Beyotime Institute of Biotechnology) to assure equal protein loading, blocked for 1 h with 5% powdered non-fat dry milk in 25 mM Tris-HCl (pH 8.0), 144 mM NaCl and 0.1% Tween 20 (TBS-T), and incubated overnight at 4C with the following primary antibodies: PPAR-, PPAR-, NF-B P65 (cat