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Jurkat cells were transfected using Lipofectamine LTX reagent with Plus reagent (Invitrogen, 15338030) according to the manufacturers protocol

however surgical intervention may help treat the underlying cause of amblyopia development

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doi: 10.1038/s41598-023-49089-y 61 ArraMAbu-AmerY

At the present time, early screening -with biomarker panels or a liver ultrasound, ideally with transient elastography- is reserved for high-risk patients (i.e., obese patients with T2DM or elevated plasma AST/ALT levels or evidence of steatosis at a random liver exam) until more accurate non-invasive methods are available