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All animals used in this study were anesthetized with inhalational isoflurane (induction at 4.0% and maintenance at 2.0%) prior to experimental procedures and, when required, were subsequently euthanized by cervical dislocation prior to analysis

The ubiquitin ligase RNF8 initiates the ubiquitination of H2AX, and RNF168 amplifies the ubiquitination response, which triggers the recruitment of p53-binding protein 1 (53BP1) and Rap80/BRCA1 to the lesion [92, 93]

And, as a natural part of the aging process, cellular production of Glutathione begins to decline at the age of 20

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