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Subsequently, 180 l fresh culture medium and 20 l of samples (0.00585800 M in sterile water) were added per well and incubated for 30 minutes

This was numerically smaller than the MCAO + Vehicle group, which had an infarct volume of 24.1 4.6%

YW: Conceptualization, Formal Analysis, Funding acquisition, Validation, Visualization, Writing review and editing

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The proteins were visualized by exposure machine (GE, Amersham Imager 680) using Pierce ECL Plus Western Blotting Substrate (Thermo Fisher Scientific, 32132) and the protein bands were quantitatively analyzed with ImageJ software

To our knowledge, this is the first study to demonstrate that SYNCRIP regulates both the transcription and mRNA stability of SIRT1