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Sublingual or liquid B12 forms offer enhanced absorption and are often favored for immediate effect

On day 2, tissues were washed again using 0.1 M PBS, incubated in secondary antibody diluted by blocking solution for 2 h at room temperature, covered to prevent light exposure, washed again, and cover-slipped using DAPI Fluoromount G (Southern Biotech) or Fluoromount G (Southern Biotech)

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Optimum preparation is with blender or shaker

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