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The main fermentation was conducted in 100 mL of YCK50D medium (Cha et al., 2004) containing 30 g/L yeast extract, 5 mM cysteine, 0.6 g/L KH 2 PO 4 , and 50 g/L glucose

Estrogen decline can affect gut integrity and the microbiome, sometimes leading to bloating, food sensitivities, or leaky gut. A 2010 animal study found BPC-157 protected against gut lesions and accelerated healing in the stomach and intestines

Therefore, these histological abnormalities have the potential to negatively impact the humoral immune function in hens following exposure to OTA

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