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Finally, the mixture of both proteins was applied to the -CD affinity column and the chromatography was conducted as described above, including the UV illumination step at 355 nm for the elution of the Azo-tagged Azurin after the mScarlet protein had been washed out

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However, factors such as poor diet, environmental pollution, chronic stress and ageing can compromise the body's ability to produce and maintain adequate glutathione levels