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J Alzheimers Dis 75(3):959969

With no airspace in the IV line, you can start inserting the cannula into the patients vein

aCSF was removed by careful suctioning and replaced with aCSF containing vehicle (aCSF+aCSF), 100 ng/ml with carrier free adult recombinant Hepatocyte Growth Factor (HGF) (R and D Systems, MN, USA) in aCSF, 10 10 M Hinge (Harding lab), 50 ng/ml in aCSF, 10 10 M Dihexa (Harding lab) in aCSF, 10 12 M Dihexa in aCSF or 50 ng/ml HGF+10 12 M Dihexa in aCSF for 30 minutes at 37 C

Drug displaced from plasma protein will of course distribute throughout the volume of distribution, so that a 5% increase in the amount of unbound drug in the body produces at most a 5% increase in pharmacologically active unbound drug at the site of action