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The next morning, the overnight culture was subsequently diluted 100:1 in fresh LB medium supplemented with the same antibiotics and incubated for 23 h until reaching an optical density at 600 nm (OD 600 = 0.40.5)

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Cholinergic urticaria Contact urticaria Auto-immune aetiology Treatment of chronic urticaria: Non-sedating antihistamines daily for 6 months If standard dose is ineffective titrate up to 4 fold the dose over one to two weeks Sedating antihistamines: additionally at night if sleep disturbed e.g

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