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Prime editing, which employs a Cas9 nickase fused to reverse transcriptase along with a prime editing guide RNA, enables installation of all possible base transitions as well as small insertions and deletions without inducing DSBs

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10.5713/ajas.2011.11273 46 ZhuF.ZhaoX.LiJ.GuoL.BaiL.QiX

Prandial timing matters more for gastric modulators (amylin analogues, GLP-1 agonists) than for systemic metabolic agents