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Subsequently, the pellets were resuspended in RPMI 1640 and then layered on a Percoll density gradient as described previously 44 to isolate either LPMCs or TILs

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[DOI] [PubMed] [Google Scholar] Horvth T., Bartos C., Bocsik A., Kiss L., Veszelka S., Deli M., et al

Novel bispecific nanobody mitigates experimental intestinal inflammation in mice by targeting TNF-alpha and IL-23p19 bioactivities

We, therefore, modeled the majority part of the c-MET extracellular domain (all domains except for IPT3 and IPT4) as well as the majority part of HGF (all domains except for K3 and K4) by using the cryo-EM structure of sub-complex that was determined at higher resolution, with the help of the available crystal structures of different domains of c-MET and HGF 14,24,25 (Supplementary Fig