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666,667 PDK1 inhibits the activity of the PDH complex through phosphorylation, reducing the oxidation of pyruvate to acetyl-CoA in the Krebs cycle and increasing lactate production

Briefly, 10 ml of cells was pelleted in the presence of 0.005% Tween 20 and fixed by resuspending in 10 ml of ethanol/acetic acid (3:1) at RT for 1 h

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We used these following antibodies: anti-CD11b (BD Biosciences, M1/70 # 557657), CD11c (BioLegend, N418 # 117338, BD Biosciences, HL3 #553800), Ly6G (BD Biosciences, 1A8 # 563979), CD115 (eBioscience, AFS98 # 46-1152-82), Ly-6C (BioLegend, HK 1.4#128006), CD19 (BD Biosciences, 1D3 # 563148), MHC class II (BioLegend, M5/114.15.2 # 107620), CD64 (BD Biosciences, X54-5/7.1 # 558455), F4/80 (BioLegend, BM8#123114), CD45.1 (BioLegend, A20 #110730), CD45.2 (BioLegend,104 # 109820, BD Biosciences, 104 # 560693), CCR2 (R&D Systems, # FAB5538A) and streptavidin (BD Biosciences, #563260, 563261)

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