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The ATP produced by the mitochondria is used to produce NADPH in a 1:1 stoichiometry, and the concentration of NADPH is estimated using an NADPH standard curve (MP Biomedicals 264671-1) (Fig

doi: 10.1074/jbc.M110.158790

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The DIA MS/MS spectra are generally much more complex than DDA-MS/MS, and therefore the identifications by dDIA pseudo-MS/MS rely more on fragment ions of higher abundance