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Using linear mixed-effects models, after adjusting for age, sex, BMI, tumor, cohort, date of stool sampling, patient ID (random effect) and pooling all time points of stool collection during immunotherapy ( n = 84 patients, 165 feces), we found that stool histidine (alongside other essential amino acids) inversely correlated with the occurrence of a severe irAEs (grade 3) (Extended Data Fig

doi:10.1038/s41598-020-80171-x

Because biological functions might differ significantly among cells, bulk assessments of pooled cells are unable to distinguish between the diverse functions of different populations

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