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Matloubian, M., Somasundaram, T., Kolhekar, S

Isolation of WAT stromal vascular cells and adipocyte differentiation To isolate the stromal vascular fraction, minced iWAT (combined depots from 45 mice per genotype) from 46-week-old mice was digested in 1 mg ml 1 collagenase D (Roche) with 1.5% BSA in sterile Hanks Balanced Salt Solution and incubated in a 37 C shaking water bath for 1 h

For measurement of total glutathione, the cell pellets were resuspended in 500 l of ice-cold 5% [w/v] 5-sulfo-salicylic acid dehydrate (SSA) and homogenized by vortexing in the presence of 300 L zirconium beads (BioSpec Products) at 4C for 30 min

Lactobacillus casei ATCC 393 and its metabolites alleviate dextran sulphate sodium-induced ulcerative colitis in mice through the NLRP3-(Caspase-1)/IL-1 pathway

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asiatica play an important role in antitumor effects, especially benzophenanthridine alkaloids