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Fixed cells were permeabilized with saponin (0.5% in PBS) for 10 min, washed with PBS, and stained with Alexa Fluor 647 Phalloidin (1/50

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Furthermore, new research suggests that exercise and dietary assistance before pregnancy may be more effective than throughout pregnancy at promoting a healthy placentation and fetal growth (Potdar, A, & M, 2014)

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