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Measurement of serum AST and ALT levels Serum AST and ALT levels were measured using the Micro Glutamic-oxalacetic Transaminase (GOT) Assay Kit (Solarbio, BC1565) and Glutamic-pyruvic Transaminase (GPT) Activity Assay Kit (Solarbio, BC1555) in accordance with the manufacturers instructions

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The complex atomic model was refined through iterative rounds using Coot 64 and phenix.refine 65

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Using human liver microsomes, it was also recently reported that sulforaphane metabolites (0-200 M) had little-to-no effect on the activities of CYP1A2, CYP2B6, CYP2D6, and CYP3A4 (23)