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Sulfur makes up 0.25% of human body weight and occurs widely in nature, being present in every cell of both animals and plants

doi: 10.1111/j.1365-2567.2005.02181.x 59 Cubillos-RuizJRSilbermanPCRutkowskiMRChopraSPerales-PuchaltASongMet al

Both excess and deficiency of THs quickly lead to significant changes in body weight: in hypothyroidism, total body weight can increases even by one-third, while in hyperthyroidism, it can decrease by approximately 15%

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doi: 10.1152/ajplung.00362.2023 157 ZhouKWenQZuoYBaiGSunR

To prevent precipitation CBS FL-CHis and its mutants were buffer exchanged into 20 mM HEPES, pH 7.4, 0.01% triton X-100 whereas CBS RD was exchanged into 20 mM HEPES, pH 7.4, 200 mM NaCl