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These cultures contain mainly astrocytes and only low amounts of contaminating oligodendrocytes and microglial cells [53, 54]

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It was identified through an extensive medicinal chemistry programme focused on compounds lacking the central Ala-Trp dipeptide of GHRP-1, ultimately yielding a compound with a distinct and highly specific pharmacological profile

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Cell pellets were resuspended in LDOX lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 0.01 mg ml 1 DNAseI) to an OD 600 of 140 and lysed by two rounds of sonication for 2 min at 50% amplitude with 2 s intervals using a Sonopuls (Bandelin) sonicator

This study aimed propose a survival tree models that could help to predict mortality after paediatric HSCT in a cohort of high risk leukemia patients