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Drug solutions, typically 5 mg/ml in HPLC-grade water (except for Dihexa, prepared in DMSO), were added to rat serum, and the mixture was incubated at 37C

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However, given the putative importance of neuroinflammation in this condition, we believe that the ME/CFS neuroimaging field could benefit from basing a priori targeting of brain regions of interest in the newly emerging human neuroinflammation literature recently meta-analyzed by Kraynak et al

doi: 10.1152/ajpgi.00324.2001

The same procedures were applied to the control group but this time saline was used

Also, there is increasing interest in potential targets for TXNRD1-based cancer therapy