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The author claimed that the position of the last double bond is more critical for induction of ferroptosis than the degree of unsaturation

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As demonstrated recently by Liu et al ., an increase in labile iron was required for ferroptosis triggered by cysteine deficiency but not direct inhibition of GPX4 [51]

Next, the membrane was incubated with electrochemiluminescence substrate (#1705060, Bio-Rad, Hercules, CA, USA) for 5 min and analyzed using FluorChem M (ProteinSimple, Santa Clara, CA, USA)

The cell suspension was subsequently transferred to a flat-bottomed 96-well plate, with 200 l per well

Other than that everybody is going to get different results, everybody