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The test started when the participant initiated the first standing motion

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Following DNA end repair and preparation, samples were subjected to a clean-up with MAGBIO beads (MAGBIO Genomics Inc, USA), prior to adapter ligation, as per ONT recommendations

To assess the disparities between groups, statistical comparisons were performed employing one-way ANOVA, the KruskalWallis H test and Pearsons 2 test, as appropriate for the specific data type

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It is important to highlight that metabolic syndrome and its components are strongly associated with NAFLD/MASLD (20)