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Moreover, evidence is lacking for coupling between ferroptosis in astrocytes or cardiac fibroblasts and secondary injury to parenchymal cells, limiting interpretation of the coordinated progression of neurologic and cardiac damage

The cell suspension was centrifuged at 700 g for 10 min at 4 C, then the supernatant was transferred to a new 2 mL tube and centrifuged at 12,000 g for 15 min at 4 C