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The GATA4 binding proteins, including p300, RACK1, CDK9, TBX5, Nkx2.5, MEF2, SRF, NFATc4, and HAND2, bind with the C-terminal zinc finger domain of GATA4 (residues 256-326), which includes the multimerization region [13, 14, 17, 37, 44, 45, 66], suggesting that numerous cofactors are concentrated near this region

The cells were seeded in a 6-well plate (Corning) and transfected with the constructs of rat wild-type Tshr ( Tshr WT -GFP ) and truncated Tshr ( Tshr Df -GFP ) in pAcGFP1-N1 (Clontech, 632469) backbone (The cells transfected with GFP expression vector pAAV-GFP was used as negative control)

4.3.1.2 Common detectors for GC There are many selective detectors such as ECD, nitrogenphosphorous detector (NPD), flame photometric detector (FPD), and flame ionization detector (FID)

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