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Intracellular GSH is further lowered by taking high dose TTFD, and there is not enough enzyme activity to recycle it back Oxidative stress is made worse In the above scenario, taking a high dose of TTFD may not be appropriate

Bartness TJ, Liu Y, Shrestha YB, Ryu V (2014) Neural innervation of white adipose tissue and the control of lipolysis

The tissue extracts were diluted as necessary with acetonitrile/DI water to fit the calibration range

Meanwhile, knockdown of REST significantly increased GSH synthesis, restored GPX4 expression, and ultimately attenuated ferroptosis, which had an additive effect on the ferroptosis inhibitors Fer-1 and DFO