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Besides activation through pattern recognition receptors, NF-B is also regulated by pro-inflammatory cytokines such as tumor necrosis factor (TNF)- and interleukin-1 (IL-1) (Yu et al., 2020), leading to the transcription of inactive pro-IL-18 and pro-IL-1 (Wang et al., 2023b)

The presence of multiple amide and amine functionalities supports protonation under positive ion mode conditions

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