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Alpha agonists

While all of these elution methods are relatively harsh, this is the most extreme and destroys any functional antibodies, so this is not a good method when wanting to use antibodies for a downstream purpose

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For longer-term storage, refrigeration or freezer storage may be appropriate depending on laboratory protocols

The concentration of protein-bound thiols was equal to the concentration of total thiols and disulfides minus the concentration of total small molecule thiols and disulfides, as shown in Fig

doi: 10.1080/20013078.2019.1688936 19