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In contrast to the GSSCys assay, the k cat app / K m app values highly depended on the substrate concentration and differed from the 1/ values by up to one order of magnitude

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Nature 573 , 230234 (2019)

Consequently, pCB formulations emphasize a low oral bioavailability that led to the necessity of other administration routes to improve pCBs bioavailability and efficacy, such as the local (topical), transcutaneous (transdermal), pulmonary, and transmucosal routes [25] that include intranasal and rectal routes, with the last one demonstrating double the rate of bioavailability compared to the oral route [22]

It can be synthesised in many tissues throughout the body, varying from the central nervous system, liver, kidney, cardiovascular system, lungs and within the gastrointestinal tract