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doi: 10.1038/s41418-019-0299-4 197 OparaugoNCOuyangKNguyenNPNNelsonAMAgakGW

Yin, X., et al

8 Streptozotocin (STZ), a glucose analog derived from Streptomyces achromogenes , selectively targets and destroys pancreatic beta cells in rodent models, serving as a widely utilized tool for inducing experimental diabetes

Proteins were precipitated again and digested with LysC and trypsin in 200 mM EPPS buffer pH 8.0 at a 100:1 substrate-to-enzyme ratio overnight at 37C