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Considering that very little is known about the local and temporal concentrations of NADPH and TGR and the multiple redox-associated conformational states, it is difficult to assess how accurately the biochemical assay with recombinant TGR models the inhibition of TGR in worms ex vivo or in vivo

In addition, the observed side-chain flexibility of V12 and F112 indicated that these residues are mainly involved in the overall shaping of the active site and help to position the flavonoid substrate in accordance with the presented structural evidence

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References Salazar-Gmez A et al (2023) Medicinal plants used for eye conditions in Mexicoa review

Figure 7 shows the mass spectrum of Ni-citrate [M.L 2 -H] + from P

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